Development of SiN nanopore sensors for direct DNA sequencing
Itaru YANAGI1, Rena AKAHORI1, Yusuke GOTO1, Kenichi TAKEDA1
This paper summarizes the latest developments regarding solid-state nanopores achieved in our laboratory. To realize DNA sequencing with solid-state nanopores, many issues must be resolved. In particular, from the standpoint of device fabrication and DNA manipulation, we believe that there are three key issues: (1) stable fabrication of a small nanopore with a diameter on the same order as that of DNA, (2) fabricating an ultrathin membrane, and (3) decreasing the DNA translocation speed through a nanopore. We addressed these three issues, and successfully observed sequence-specific currents through a nanopore while DNA remained in the nanopore.
- 1 Hitachi Ltd, Research and Development Group, Biosystems Research Dept