Super-resolution microscopy having both high spatial and high temporal resolution
In the field of biology, there are high expectations for super-resolution microscopy, due to the higher spatial resolution than conventional microscopy being able to elucidate the functions of individual protein molecules in live samples, which could not be observed directly before. However, many of the super-resolution methods sacrifice temporal resolution by using longer exposure times so that a larger number of image frames are required for a single super-resolution image. Aiming at super-resolution without sacrificing temporal resolution, the authors have developed a spinning disk super-resolution microscopy, which achieves twice the conventional resolution with single frame acquisition. In this article, the principles of the newly developed super-resolution microscopy and the similarity to structured illumination microscopy are explained.
- 1 Optical System Development Dept. 2, Olympus Corporation