Bioimaging: Super-resolution microscope, stimulated emission depletion (STED)
Stimulated emission depletion (STED) microscopy is one of the fluorescence microscopy techniques, which enables sub-diffraction imaging of biological samples. The principle of the STED microscope and the role of optical components are explained. The alignment procedure and sample preparations are also discussed, and examples of STED images of fluorescent beads and actin filaments are demonstrated. Perspectives of the application of STED microscopy are also discussed.
- 1 Graduate School of Science and Engineering, Yamagata University