Next-generation in vivo fluorescence imaging for deep tissue in the second near-infrared optical window
Yasutomo NOMURA1,2, Hiro YAMATO1, Tatsuto IIDA1, Takashi JIN2
The second near-infrared window ranges between 900 and 1400 nm, namely NIR-II, and has advantages for deep tissue imaging with extrinsic fluorophores due to the lack of autofluorescence, low light absorption, and reduced scattering. When quantum dots (QDs) having fluorescence in NIR-II were introduced into the tail vein of mice, we clearly observed the cerebral vein through the intact skull. Then QDs were moved from blood to the liver and kidney. When QDs were injected transcutaneously from the hindlimb, a strong fluorescence was observed in the popliteal and iliac lymph nodes. Changes in fluorescence were analyzed using the compartment model. As a new application, we plan to monitor electrical activity in living tissue noninvasively using membrane potential dye with NIR-II fluorescence.
- 1 Department of Systems Life Engineering, Maebashi Institute of Technology
- 2 RIKEN, Quantitative Biology Center