OYO BUTURI Vol.87 No.5 (2018)
Fundamental Lecture

Construction of an optical system (Practical): Let's build a confocal laser scanning microscope (CLSM)

Takayuki IWASAKI1

Confocal laser scanning microscopes (CLSM) have been used for high-definition luminescence observations in a wide range of fields such as physics and biology. In this article, the basic system and principle of CLSM, and the role and selection of devices are explained for beginners who want to assemble a CLSM by installing optical devices on an optical surface plate. There are different decisions to make to assemble a CLSM, such as which optical device to use and the adjustment method for the optical system, however, this article explains the minimum required devices for the construction of CLSM.

  • 1 School of Engineering, Department of Electrical and Electronic Engineering, Tokyo Institute of Technology
OYO BUTURI Vol.87 No.5 p.371 (2018)